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murine recombinant leptin  (R&D Systems)


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    R&D Systems murine recombinant leptin
    Murine Recombinant Leptin, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 129 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+murine+leptin/Recombinant+Human+Leptin+Protein%2C+CF/pm41875058-43-18-24
    Average 95 stars, based on 129 article reviews
    murine recombinant leptin - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Recombinant:

    Article Title: Endocrine-exocrine signaling drives obesity-associated pancreatic ductal adenocarcinoma
    Article Snippet: .. For signaling studies, cells were grown in serum-free conditions overnight, treated with 100 ng/mL recombinant murine leptin (R&D Systems, Cat# 498-OB) in serum-free media, and lysed for protein analysis after 20 minutes (peak induction of downstream signaling). .. Viability was assessed 72 hours after treatment using the CellTiter Glo (CTG) assay (Promega).

    Article Title: Daily leptin blunts marrow fat but does not impact bone mass in calorie-restricted mice
    Article Snippet: .. At 18:00 h daily, all mice received subcutaneous injection of recombinant murine leptin (R&D Systems) at 1 mg/kg/day (LEP1), leptin at 2 mg/kg/day (LEP2), or vehicle (VEH) (N = 6–8/group). ..

    Article Title: Astrocytes Modulate Distribution and Neuronal Signaling of Leptin in the Hypothalamus of Obese A vy Mice
    Article Snippet: .. Carrier-free recombinant murine leptin (R & D Systems, Minneapolis, MN) was conjugated with Alexa568 with a protein labeling kit from Invitrogen (Molecular Probes, Eugene, Oregon) and purified on a BioGel P10 column (Bio-Rad Laboratories, Hercules, CA, USA) as described previously ( Tu et al. 2010 ). ..

    Article Title: Transcriptome-guided GLP-1 receptor therapy rescues metabolic and behavioral disruptions in a Bardet-Biedl syndrome mouse model
    Article Snippet: .. Animals were fasted overnight (~12–14 hours) before receiving an i.p. injection 1 hour in the dark period of either 0.1% wt/vol BSA (vehicle) or recombinant murine leptin (2 mg/kg body weight; R&D Systems) and monitored for changes in food intake and body weight over 24 hours. .. Animals were fasted overnight (~12–14 hours) before receiving an i.p. injection 1 hour in the dark period of either 0.1% wt/vol BSA (vehicle) or CCK-8 sulfated (2 or 4 μg/kg body weight; Tocris Bioscience) and monitored for changes in food intake and body weight over 24 hours.

    Article Title: Leptin‐Induced Endothelial Dysfunction Is Mediated by Sympathetic Nervous System Activity
    Article Snippet: .. Ten micrograms of recombinant murine leptin (R&D Systems, Inc) was injected subcutaneously every other day for a total of 4 doses. ..

    Article Title: Transcriptome-guided GLP-1 receptor therapy rescues metabolic and behavioral disruptions in a Bardet-Biedl Syndrome mouse model
    Article Snippet: .. Leptin: Animals were fasted overnight (~12 to 14 h) before receiving 6 an i.p. injection 1 h in the dark period of either 0.1% w/v bovine serum albumin (vehicle) 7 or recombinant murine leptin (2 mg/kg of body weight; R&D Systems, Minneapolis, MN, 8 USA) and monitored for changes in food intake and body weight over 24 h. 9 Cholecystokinin-8: Animals were fasted overnight (~12 to 14 h) before receiving an i.p. 10 injection 1 h in the dark period of either 0.1% w/v bovine serum albumin (vehicle) or CCK-11 8 sulfated (2 or 4 μg/kg of body weight; Tocris Bioscience, Minneapolis, MN, USA) and 12 monitored for changes in food intake and body weight over 24 h. Exendin-4: Animals were 13 fasted overnight (~12 to 14 h) before receiving an i.p. injection1 h in the dark period of 14 either 0.1% w/v bovine serum albumin (vehicle) or GLP-1 receptor agonist, exendin-4 15 (0.1, 1 or 2 μg/kg of body weight; Tocris Bioscience, Minneapolis, MN, USA) and 16 monitored for changes in food intake and body weight over 24 h. 17 18 Semaglutide Study. .. To determine the therapeutic potential of GLP-1R agonist on body 19 composition, food intake, and glucose tolerance, mature animals (18-30 weeks old) were 20 subcutaneously injected with either vehicle (6%v/v DMSO in 0.9% saline) or semaglutide 21 (0.15 mg/kg of body weight; gift from Novo Nordisk, Denmark) in a crossover study design 22 (Figure 5A).

    Injection:

    Article Title: Daily leptin blunts marrow fat but does not impact bone mass in calorie-restricted mice
    Article Snippet: .. At 18:00 h daily, all mice received subcutaneous injection of recombinant murine leptin (R&D Systems) at 1 mg/kg/day (LEP1), leptin at 2 mg/kg/day (LEP2), or vehicle (VEH) (N = 6–8/group). ..

    Article Title: Transcriptome-guided GLP-1 receptor therapy rescues metabolic and behavioral disruptions in a Bardet-Biedl syndrome mouse model
    Article Snippet: .. Animals were fasted overnight (~12–14 hours) before receiving an i.p. injection 1 hour in the dark period of either 0.1% wt/vol BSA (vehicle) or recombinant murine leptin (2 mg/kg body weight; R&D Systems) and monitored for changes in food intake and body weight over 24 hours. .. Animals were fasted overnight (~12–14 hours) before receiving an i.p. injection 1 hour in the dark period of either 0.1% wt/vol BSA (vehicle) or CCK-8 sulfated (2 or 4 μg/kg body weight; Tocris Bioscience) and monitored for changes in food intake and body weight over 24 hours.

    Article Title: Leptin‐Induced Endothelial Dysfunction Is Mediated by Sympathetic Nervous System Activity
    Article Snippet: .. Ten micrograms of recombinant murine leptin (R&D Systems, Inc) was injected subcutaneously every other day for a total of 4 doses. ..

    Article Title: Transcriptome-guided GLP-1 receptor therapy rescues metabolic and behavioral disruptions in a Bardet-Biedl Syndrome mouse model
    Article Snippet: .. Leptin: Animals were fasted overnight (~12 to 14 h) before receiving 6 an i.p. injection 1 h in the dark period of either 0.1% w/v bovine serum albumin (vehicle) 7 or recombinant murine leptin (2 mg/kg of body weight; R&D Systems, Minneapolis, MN, 8 USA) and monitored for changes in food intake and body weight over 24 h. 9 Cholecystokinin-8: Animals were fasted overnight (~12 to 14 h) before receiving an i.p. 10 injection 1 h in the dark period of either 0.1% w/v bovine serum albumin (vehicle) or CCK-11 8 sulfated (2 or 4 μg/kg of body weight; Tocris Bioscience, Minneapolis, MN, USA) and 12 monitored for changes in food intake and body weight over 24 h. Exendin-4: Animals were 13 fasted overnight (~12 to 14 h) before receiving an i.p. injection1 h in the dark period of 14 either 0.1% w/v bovine serum albumin (vehicle) or GLP-1 receptor agonist, exendin-4 15 (0.1, 1 or 2 μg/kg of body weight; Tocris Bioscience, Minneapolis, MN, USA) and 16 monitored for changes in food intake and body weight over 24 h. 17 18 Semaglutide Study. .. To determine the therapeutic potential of GLP-1R agonist on body 19 composition, food intake, and glucose tolerance, mature animals (18-30 weeks old) were 20 subcutaneously injected with either vehicle (6%v/v DMSO in 0.9% saline) or semaglutide 21 (0.15 mg/kg of body weight; gift from Novo Nordisk, Denmark) in a crossover study design 22 (Figure 5A).

    Labeling:

    Article Title: Astrocytes Modulate Distribution and Neuronal Signaling of Leptin in the Hypothalamus of Obese A vy Mice
    Article Snippet: .. Carrier-free recombinant murine leptin (R & D Systems, Minneapolis, MN) was conjugated with Alexa568 with a protein labeling kit from Invitrogen (Molecular Probes, Eugene, Oregon) and purified on a BioGel P10 column (Bio-Rad Laboratories, Hercules, CA, USA) as described previously ( Tu et al. 2010 ). ..

    Purification:

    Article Title: Astrocytes Modulate Distribution and Neuronal Signaling of Leptin in the Hypothalamus of Obese A vy Mice
    Article Snippet: .. Carrier-free recombinant murine leptin (R & D Systems, Minneapolis, MN) was conjugated with Alexa568 with a protein labeling kit from Invitrogen (Molecular Probes, Eugene, Oregon) and purified on a BioGel P10 column (Bio-Rad Laboratories, Hercules, CA, USA) as described previously ( Tu et al. 2010 ). ..



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    Criteria for chosen guide RNAs for <t> Leptin </t> KO. Score was computed as 100 % minus a weighted sum of off-target hit-scores in the target genome (full ranked list not shown). Single nucleotide polymorphism (SNP) NA indicates no common SNP found in the gRNA. <t> Leptin </t> KO iPSCs were then differentiated into <t> PDiPSCs </t> using the micromass procedure as described above.
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    Image Search Results


    Criteria for chosen guide RNAs for Leptin KO. Score was computed as 100 % minus a weighted sum of off-target hit-scores in the target genome (full ranked list not shown). Single nucleotide polymorphism (SNP) NA indicates no common SNP found in the gRNA. Leptin KO iPSCs were then differentiated into PDiPSCs using the micromass procedure as described above.

    Journal: European cells & materials

    Article Title: DESIGNER FAT CELLS: ADIPOGENIC DIFFERENTIATION OF CRISPR-CAS9 GENOME-ENGINEERED INDUCED PLURIPOTENT STEM CELLS

    doi: 10.22203/eCM.v046a09

    Figure Lengend Snippet: Criteria for chosen guide RNAs for Leptin KO. Score was computed as 100 % minus a weighted sum of off-target hit-scores in the target genome (full ranked list not shown). Single nucleotide polymorphism (SNP) NA indicates no common SNP found in the gRNA. Leptin KO iPSCs were then differentiated into PDiPSCs using the micromass procedure as described above.

    Article Snippet: Leptin knockout PDiPSCs in culture were supplemented with 1,000 ng/mL of murine leptin (498-OB-05M; R&D Systems, Minneapolis, MN, USA), a concentration that was determined after performing a leptin dose analysis.

    Techniques:

    Leptin KO PDiPSCs were cultured in expansion or adipogenic media for 14 days. ( a ) Timeline of culture conditions. ( b ) All groups were stained with Oil Red O and Days 5, 9, and 14 are shown here. Red stain indicates lipid content in cells. The yellow arrows point to lipid-containing cells. Scale bar is 200 μm. ( c ) All Groups were stained with BODIPY/DAPI BODIPY, green, stains for lipids and DAPI, blue, stains nuclei. Scale bar is 100 μm. ( d ) WT and leptin KO PDiPSCs were collected at various timepoints for gene expression characterization. Pparg, Adip , and Lep , were target genes evaluated. Values represent fold change ± SEM ( n = 3 ). Letters represent significance (A p < 0.05, WT adipogenic media; B p < 0.05 leptin KO expansion media; C p < 0.05 leptin KO adipogenic media) from WT PDiPSCs expansion media control. In an effort to restore adipogenic differentiation, murine leptin was added to the culture media as a supplement. ( e ) Leptin KO PDiPSCs with leptin added were cultured in expansion or adipogenic media and for 14 days and were stained with Oil Red O and days 5, 9, and 14 as shown here. Scale bar is 200 μm. ( f ) The same groups as ( e ) were stained with BODIPY/DAPI. Scale bar is 100 μm. ( g ) Lipid content of the PDiPSCs (leptin KO and leptin KO with leptin added) was measured at day 14 using the BODIPY images. Bars represent lipid content (%) ± SEM ( n = 3 ). Asterisks represent significance (* p < 0.01) compared with the leptin KO PDiPSCs in adipogenic media. ( h ) Cells from leptin KO with and without leptin added were collected at various timepoints for gene expression characterization, day 9 is shown here. Pparg, Apn , and Lep , were target genes evaluated. Values represent fold change ± SEM ( n = 3 ). Asterisks represent significance (** p < 0.0001 , *** p < 0.00001) compared with the leptin KO PDiPSCs expansion media control. ( i ) For 9 days, Leptin KO PDiPSCs were treated with adipogenic media and supplemented with varying doses of leptin. Samples were fixed and stained with Oil Red O. 1,000 ng/mL of leptin added to culture media displays the highest concentration of lipid-containing cells. Scale bar is 200 μm. All statistics were run using a 2-way ANOVA with Sidak’s post-hoc test. SEM, standard error of the mean.

    Journal: European cells & materials

    Article Title: DESIGNER FAT CELLS: ADIPOGENIC DIFFERENTIATION OF CRISPR-CAS9 GENOME-ENGINEERED INDUCED PLURIPOTENT STEM CELLS

    doi: 10.22203/eCM.v046a09

    Figure Lengend Snippet: Leptin KO PDiPSCs were cultured in expansion or adipogenic media for 14 days. ( a ) Timeline of culture conditions. ( b ) All groups were stained with Oil Red O and Days 5, 9, and 14 are shown here. Red stain indicates lipid content in cells. The yellow arrows point to lipid-containing cells. Scale bar is 200 μm. ( c ) All Groups were stained with BODIPY/DAPI BODIPY, green, stains for lipids and DAPI, blue, stains nuclei. Scale bar is 100 μm. ( d ) WT and leptin KO PDiPSCs were collected at various timepoints for gene expression characterization. Pparg, Adip , and Lep , were target genes evaluated. Values represent fold change ± SEM ( n = 3 ). Letters represent significance (A p < 0.05, WT adipogenic media; B p < 0.05 leptin KO expansion media; C p < 0.05 leptin KO adipogenic media) from WT PDiPSCs expansion media control. In an effort to restore adipogenic differentiation, murine leptin was added to the culture media as a supplement. ( e ) Leptin KO PDiPSCs with leptin added were cultured in expansion or adipogenic media and for 14 days and were stained with Oil Red O and days 5, 9, and 14 as shown here. Scale bar is 200 μm. ( f ) The same groups as ( e ) were stained with BODIPY/DAPI. Scale bar is 100 μm. ( g ) Lipid content of the PDiPSCs (leptin KO and leptin KO with leptin added) was measured at day 14 using the BODIPY images. Bars represent lipid content (%) ± SEM ( n = 3 ). Asterisks represent significance (* p < 0.01) compared with the leptin KO PDiPSCs in adipogenic media. ( h ) Cells from leptin KO with and without leptin added were collected at various timepoints for gene expression characterization, day 9 is shown here. Pparg, Apn , and Lep , were target genes evaluated. Values represent fold change ± SEM ( n = 3 ). Asterisks represent significance (** p < 0.0001 , *** p < 0.00001) compared with the leptin KO PDiPSCs expansion media control. ( i ) For 9 days, Leptin KO PDiPSCs were treated with adipogenic media and supplemented with varying doses of leptin. Samples were fixed and stained with Oil Red O. 1,000 ng/mL of leptin added to culture media displays the highest concentration of lipid-containing cells. Scale bar is 200 μm. All statistics were run using a 2-way ANOVA with Sidak’s post-hoc test. SEM, standard error of the mean.

    Article Snippet: Leptin knockout PDiPSCs in culture were supplemented with 1,000 ng/mL of murine leptin (498-OB-05M; R&D Systems, Minneapolis, MN, USA), a concentration that was determined after performing a leptin dose analysis.

    Techniques: Cell Culture, Staining, Gene Expression, Control, Concentration Assay